ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (8): 1617-1624.doi: 10.11843/j.issn.0366-6964.2018.08.006

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The Investigation to Expression of Key Genes for Muscle Development before and after Long-term High Loading Exercise in Mongolian Horses

BAI Dong-yi1, Suriga1, DUGARJAVIIN Mang-lai1*, Wuyihan2, ZHAO Yi-ping1, LI Bei1, BOU Gerelchimeg1, ZHANG Xin-zhuang1, TAO Ke-tao1, ZHAO Ruo-yang1, Tugeqin1, QING Bai1, Xurenqimuge1   

  1. 1. Equine Research Center, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Inner Mongolia Center for Disease Comprehensive Control and Prevention, Hohhot 010030, China
  • Received:2017-11-21 Online:2018-08-23 Published:2018-08-23

Abstract:

The aims of this study were to compare expression quantity of key genes for muscle development before and after long-term high loading exercise in Mongolian horse, and to provide data support for the development of horse endurance training programs.Three female Mongolian horses of 5-years-old were used and continuous high loading endurance exercise were carried out for continue 4 months, and the gluteus medius were collected before and after exercise, respectively. RT-qPCR and Western blot were used to analyze the expression quantity of MYL-2 and TNNC1 genes at transcription and translation levels before and after long-term high loading exercise in Mongolian horses. The results showed that the expression quantity of MYL-2 and TNNC1 genes in Mongolian horses muscle after exercise were significantly higher than that before exercise at mRNA level(P<0.01). The expression quantity of MYL-2 significantly increased after exercise(P<0.01), the expression quantity of TNNC1 increased after exercise at protein level(P>0.05). The long-term high loading exercise promote the expression of MYL-2 and TNNC1 genes related to Mongolian horses muscle development. It is important to form endurance of Mongolian horse.

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